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    SDS-PAGE Product.

    SDS-PAGE

    SDS-PAGE Product.

    HIGH One-Step Acrylamide Solution kit

    Brand Chembio
    Cat No. CBHL-1000
    Cat No. CBHH-1000

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    HIGH One-Step Acrylamide Solution kit

     

    SDS-PAGE, which is most widely used in electrophoresis for protein separation today, is a method of separating two gels with different pH compositions into discontinuous buffer system.

    This method has the advantage of stacking proteins using the potential difference between Cl- and glycine ions generated at pH6.8 even if many samples are loaded. 

    However, the disadvantage of a maintaining a high pH level is that the glycine ion must be charged strongly with negative charges in the separating gel to separate proteins. 

    In these alkaline states, disulfide bond dissociated by deamination, alkylation or reducing agent(like DTT, β-mercaptoethanol) are reoxidated, affecting protein molecular weight.

    One-Step Acrylamide solution is a product that compensates for these shortcomings and minimizes protein changes by maintaining gel and running buffers in an acidic state and inhibits disulfide binding to Cysteine.


    Features 

    1.     Acid: One-Step Acrylamide Gel pH6.3 & One-Step Running Buffer pH7.0-7.3
    -Inhibit protein Deamination, Alkylation & Cystein Oxidation
    -Long term Gel storage

    2.     Buffer System that maintains a constant loading

    3.     Add reducing agent to One-Step Running Buffer to maintain the reducing state

    4.     Low cost
    -Get One-Step Acrylamide Gel(500ml), 20x One-Step Running Buffer, 200x Reducing agent, APS, TEMED at just 1 bottle of 30% Acrylamide (29:1, 500ml)
    -No need to purchase other reagents for Gel electrophoresis

    5.     Convenient use and Save time
    -Add the kit component APS, TEMED to complete gel
    -No need Stacking gel
    -Easily separate other size proteins by changing Only the Running Buffer
    -1.2 times fast than normal SDS-PAGE

    6.     Easily get hard to separate molecular weight in normal SDS-PAGE
    -Separate protein above 300kDa
    -Separate protein less than 15kDa

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    Ordering Information 

    Cat. No.

    Product

    Size

    CBHL-1000

    HIGH One-Step Acryl solution with LM Running Buffer

    1 Kit

    Components

     

    HIGH One-Step Acrylamide Solution (CBHA-0500)

    500ml

     

    20x Running Buffer LM (CBRL-0500)

    500ml

     

    200x Reducing Agent (CBRA-0500)

    40ml

     

    Ammonium Persulfate

    1g/10ml

     

    TEMED

    1ml

    CBHH-1000

    HIGH One-Step Acryl solution with HM Running Buffer

    1 Kit

    Components

     

    HIGH One-Step Acrylamide Solution (CBHA-0500)

    500ml

     

    20x Running Buffer HM (CBWA-0500)

    500ml

     

    200x Reducing Agent (CBRA-0500)

    40ml

     

    Ammonium Persulfate

    1g/10ml

     

    TEMED

    1ml